BC2L-A
Ca2+-dependent lectin from Burkholderia cenocepacia specific to α-mannosides (α1-2, α1-3, α1-6)
Prices
| Product number | Product | Form | Package size | In stock | Lot number | Price |
|---|---|---|---|---|---|---|
| GL-002 | BC2L-A lectin | lyophilized | 1 mg | 10 | 12854 | 160 EUR |
| 5 mg | 6 | 12854 | 720 EUR | |||
| 5x 1 mg | 2 | 12854 | 720 EUR | |||
| 10 mg | - | 12854 | 1360 EUR | |||
| bulk orders | ||||||
| Discounts may be applied for bulk orders. Biotinylated or fluorescently labeled (DyLight) variants can be provided upon request. Contact us at contact@4glyco.cz for prices and availability of those products. | ||||||
| Product Specification Sheet | .pdf 190 kB |
|---|---|
| Certificate Of Analysis (Lot Number 12854) | .pdf 247 kB |
This product is for R&D use only. Not for human or animal use.
Basic information:
| Name: | BC2L-A (BclA) |
| Organism: | Burkholderia cenocepacia |
| Expression host: | Escherichia coli |
| Tags: | no |
| Molar mass (monomer): | 13764.1 Da |
| Extinction coefficient: | 8480 M-1 cm-1 |
| Oligomeric state: | dimer |
| PDB code: | 2WRA (with Man3) |
Protein sequence:
ADSQTSSNRAGEFSIPPNTDFRAIFFANAAEQQHIKLFIGDSQEPAAYHKLTTRDGPREATLNSGNGKIRFEVSVNGKPSATDARLAPINGKKSDGSPFTVNFGIVVSEDGHDSDYNDGIVVLQWPIG
Carbohydrate specificity:
BC2L-A lectin (formerly BclA) is specific to α-mannosides. It binds d-mannose, various mannosylated oligosaccharides, and oligomannose-type N-glycans without particular preference for the linkage types (α1-2, α1-3, α1-6) [1]. BC2L-A binds heptoses (in manno- configuration) from the Burkholderia cell wall lipopolysaccharide, but with lower affinities than d-mannose and its derivatives [2].
| Ion dependency: | Ca2+-dependent |
| Glycan array data: | CFG web pages |
Stability:
Stable in a variety of buffers with pH 4.5-9.5. For example, Tris, Hepes, MES, and phosphate are suitable buffers for BCL-A. Since the presence of calcium ions in the binding site is required for lectin activity, adding calcium ions (0.1-0.5 mM CaCl2) to the working buffer is recommended. Avoid EDTA and other chelating agents as they lead to removing Ca2+ ions from the binding site and losing lectin activity. After reconstitution, the protein should be stable in the fridge for weeks. Adding sodium azide (0.02%) is recommended to avoid microbial growth.
Tm = 87 °C (DSC, 20 mM Tris, 100 mM NaCl, 0.1 mM CaCl2, pH 7.5) [1]
Applications and biological effects:
BC2L-A can be used, for example, in lectin blotting, fluorescence microscopy, flow cytometry, and lectin histochemistry experiments to study glycosylation of proteins, cells, and tissues. It can be used to isolate specifically mannosylated glycans and glycoproteins (using, e.g., lectin affinity chromatography). BC2L-A lectin is also present in commercial lectin microarrays.
In addition, BC2L-A was recently used to enrich C- and O-mannosylated peptides from the tryptic digests of cell extracts before mass spectrometry analysis [3].
References:
- Lameignere, Malinovská et al, Biochem J, 2008, doi: 10.1042/bj20071276
- Marchetti, Malinovská et al, Glycobiology, 2012, doi: 10.1093/glycob/cws105
- Hütte et al, Anal Chem, 2022, doi: 10.1021/acs.analchem.2c00742